The Applications of GC Packed Columns in Compound Purification
Chromatography is a common analysis technique based on the principle where molecules in mixture applied to the surface or into the stationary phase is separating from each other while moving with the aid of a mobile phase. The primary purpose of chromatography is to purify and extract one or more components of a sample, and its analytical goal is to determine the qualitative and quantitative chemical composition of the sample. Any chromatography technique contains three main parts: a sample, a mobile phase, and a stationary phase. The components in the mixture sample can be separated and purified depending on the different distribution rates of different components, which depends on the solubility, affinity, and interaction with the stationary phase.
The mobile phase flowing through the stationary phase can be a gas or a liquid substance. If the mobile phase is liquid, it is called liquid chromatography (LC), and if the mobile phase is a gas, it is called gas chromatography (GC). GC is applied for mixtures of gases, and volatile liquids and solid substances. LC is especially used for thermally labile and nonvolatile samples. LC can further be divided into ion exchange, separations based on size, and gel based electrophoretic techniques. A particularly common detector for GC and LC is mass spectrometry, which converts each analyte from a chemically neutral species into a positive cation, often breaking various bonds in the process. Detection of the mass of individual fragments allows determination of the chemical structure of the analyte.
In addition to LC and GC, other chromatography methods include planar and column chromatography, which depend on the shape of the stationary phase, and affinity, ion exchange, partition, adsorption, and size exclusion chromatography, which depend on the interaction between the stationary and mobile phases. Chromatography methods based on partition are very effective for the isolation and identification of small molecules such as amino acids, carbohydrates, and fatty acids. Affinity chromatography can be used to separate large molecules such as nucleic acids and proteins. Paper chromatography is used for protein separation and protein synthesis related research, while molecular-sieve chromatography is utilized to determine the molecular weight of proteins. Agarose-gel chromatography can be employed for the purification of RNA, DNA particles, and viruses. Thin layer chromatography can be used for the purification of macromolecules, the separation of complex drug ingredients, as well as the determination of pesticides.
Gas chromatography (GC) is a widely used analytical technique for separating and analyzing volatile compounds in a mixture. Packed columns were among the first types of columns used in gas chromatography before the development of capillary columns. GC packed columns are suited for separating larger compounds or when the sample size is not limited. They are often used in analyzing natural products, environmental samples, and complex mixtures.
Amerigo Scientific, a distributor focused on providing critical products and services to biomedical and life science communities, is excited to unveil its comprehensive portfolio of gas chromatography columns designed to meet the diverse separation and purification needs across various industries, including environmental science, biochemical analysis, petrochemicals, and pharmaceuticals.
With decades of experience in chromatography, Amerigo Scientific provides a diverse selection of GC packed columns, available in various dimensions, formats, stationary phases, and tubing materials, to facilitate the selection of optimal columns for a range of analytical applications. More importantly, almost all packed columns from Amerigo Scientific including NanoChrom packed columns are custom-made. From the analysis of malodorous substances, pesticide residues, and organic mercury to examining amino acids, fatty acids, and pharmaceuticals, Amerigo Scientific’s GC packed columns ensure reliable filtration, separation, and purification solutions for these industrial applications.
Amerigo Scientific is committed to providing high-quality, reliable products that enhance analytical efficiency and accuracy. With a focus on reliability and reproducibility, Amerigo’s gas chromatography solutions aim to meet the rigorous demands of modern analytical laboratories. Whether it’s for routine analysis or specialized research, Amerigo Scientific remains a trusted partner for laboratories seeking to advance their analytical capabilities and drive scientific discovery.
About Amerigo Scientific
Amerigo Scientific, as a recognized distributor in the United States, collaborates closely with leading manufacturers worldwide and invites cooperation to all companies and institutions in the branch of reagents, kits, antibodies, and many other products for life science, biochemistry, and biotechnology. Its professional team is equipped with excellent technical support and thoughtful customer service. As most of its employees have earned a graduate (Ph.D. or M.S.) degree in life science, they can comprehend customers’ questions or concerns and are always ready to provide individualized customer service of high standards.
